Penentuan Minimum Inhibitory Concentration (MIC) minyak atsiri Acorus calamus terhadap Aspergillus flavus
##plugins.pubIds.doi.readerDisplayName##
https://doi.org/10.24843/JBIOUNUD.2024.v28.i01.p09
Abstrak
Balinese lontar manuscripts are documentation of past Balinese culture. The main component of palm leaves is cellulose. Efforts to prevent damage to lontar manuscripts from cellulose-degrading fungi such as Aspergillus flavus are needed to maintain Balinese local wisdom. The purpose of this study was to evaluate the Minimum Inhibitory Concentratiton (MIC) value of jangu leaf essential oil (Acorus calamus) in inhibiting the growth of A. flavus in vitro. The results obtained were the optimum concentration of A. calamus essential oil at 5% (v/v), with an average inhibition zone diameter of 11.72 mm (strong category). Minimum Inhibitory Concentration (MIC) of jangu leaf essential oil in inhibiting A. flavus is 2% v/v with an average inhibition zone diameter of 7.08 mm. This research opens new insights in the conservation of Balinese ancient manuscripts from damage caused by cellulose-degrading fungi, by utilizing the essential oil of local Balinese plants, namely jangu leaf (A. calamus).
##plugins.generic.usageStats.downloads##
##plugins.generic.usageStats.noStats##
Referensi
Abdulmageed L. 2023. Study of morphological and physiological characteristics of some types of fungus Aspergillus Spp. Anbar Journal of Agricultural Sciences 21(2): 386–395.
Dinev T, Tzanova M, Velichkova K, Dermendzhieva D, Beev G. 2021. Antifungal and antioxidant potential of methanolic extracts from Acorus calamus, Chlorella vulgaris Beijerinck, Lemna minuta Kunth and Scenedesmus dimorphus (Turpin) Kützing. Applied Sciences (Switzerland) 11(11): 1-13.
Febia A, Mukarlina, Rahmawati. 2020. Aktivitas antifungi ekstrak metanol daun bandotan (Ageratum conyzoides) terhadap Phytophthora sp. (Im5) secara in vitro. Jurnal Protobiont 9(2): 167–174.
Fitria F, Suhartini S, dan Prihandono DS. 2023. Gambaran kapang Aspergillus Sp. pada terasi dalam kemasan tanpa merek di pasar tradisional kota Samarinda. JI-KES (Jurnal Ilmu Kesehatan) 6(2): 147–154.
Hanif A, Zamriyetti Z. 2023. Karakterisasi morfologi cendawan penyebab penyakit busuk pangkal batang pada bawang merah (Allium cepa). AGRIUM: Jurnal Ilmu Pertanian 26(1): 76–82.
Julyeda NKD, Darmayasa, IBG, Hardini J. 2021. Potential of awar-awar leaf methanol extract (Ficus septica Burm. f.) against Aspergillus niger on Balinese lontar. Metamorfosa: Journal of Biological Sciences 8(2): 268-274.
Mohammed GJ, Hameed IH. 2018. Anti-fungal, antitumor and anti-inflammatory activity of Acorus calamus. Indian Journal of Public Health Research and Development 9(3): 254–258.
Nasardin NR, Zainon M, Zulkefle A, Hanafiah MA, Ibrahim M, Rahman AI. 2018. Comparative study on steam distillation and hydro-distillation methods for agarwood oil extraction. International Journal of Applied Engineering Research 13(8): 6253–6256.
Paranatha KAD, Bratayadnya PA, dan Octaviano AL. 2022. Konservasi Naskah lontar dalam fotografi story. Retina Jurnal Fotografi 2(1): 123–132.
Parwanayoni S, Sudirga SK. 2020. Isolasi dan identifikasi senyawa antijamur daun jeringau (Acorus calamus Linn.) sebagai pengendali jamur Athelia rolfsii Sacc. penyebab penyakit busuk batang pada tanaman kedelai. Metamorfosa: Journal of Biological Sciences 7(2):10-12.
Politeo O, Popovic M, Versic Bratincevic M, Koceic P, Nincevic T, Mekinic IG. 2023. Conventional vs. microwave-assisted hydrodistillation: influence on the chemistry of sea fennel essential oil and its by-products. Plants 12(7): 32-43.
Putri D, Nasir N, Agustien A. 2019. Kemampuan jamur akar putih (Rigidoporus microporus (Swartz: Fr.) Van Ov.) dalam mendegradasi limbah selulosa. Metamorfosa: Journal of Biological Sciences 6(1): 102-114.
Rampa E, Manurun S, Sinaga H. 2023. Isolasi fungi endofit dari buah dan daun belimbing wuluh. Jurnal Penelitian Kesehatan Suara Forikes 14(1): 92–96.
Rita WS, Kawuri R, Swantara IMD. 2017. The essential oil contents of jeringau (Acorus calamus L.) rhizomes and their antifungal activity against Candida albicans. Journal of Health Sciences and Medicine 1(1): 33-41.
Rohmah IN, Alif T. 2021. Uji pengembangan spora entomopatogen bunga entomopatogen Lecanicillium lecanii menggunakan haemocytometer. Jurnal Matematika Dan Sains 1(2): 143–150.
Sharma V, Sharma R, Gautam DNS, Kuca K, Nepovimova E, Martins N. 2020. Role of vacha (Acorus calamus Linn.) in neurological and metabolic disorders: Evidence from ethnopharmacology, phytochemistry, pharmacology and clinical study. Journal of Clinical Medicine 9(4): 67-74.
Simanjuntak HA, Butar M. 2019. Uji aktivittas antifungi ekstrak etanol umbi bawang merah (Allium Cepa L.) terhadap Candida albicans dan Pityrosporum Ovale. Eksakta: Jurnal Penelitian dan Pembelajaran MIPA 4(2): 91-98.
Sintawarak P, Uthairatsamee S, Keawgrajang T. 2021. In vitro and in vivo inhibition of Cylindrocladium reteaudii by essential oils of Acorus calamus rhizomes. Environment and Natural Resources Journal 19(1): 34–42.
Szczeblewski P, Wroblewski M, Borzyszkowska-Bukowska J, Bairamova T, Górska J, Laskowski T, Samulewicz, A, Kosno, M., Sobiech, Ł, Polit JT, Kukula-Koch W. 2022. The role of centrifugal partition chromatography in the removal of β-asarone from Acorus calamus essential oil. Scientific Reports 12(1): 1–14.
Tancinova D, Maskova Z, Foltinova D, Stefanikova J, Arvay J. 2018. Effect of essential oils of lamiaceae plants on the Rhizopus spp. Journal of Food Sciences 12(1): 491–498.
Venkatesan R, Karuppiah PS, Arumugam G, Balamuthu K. 2019. β-Asarone exhibits antifungal activity by inhibiting ergosterol biosynthesis in Aspergillus niger ATCC 16888. Proceedings of the National Academy of Sciences India Section B - Biological Sciences 89(1): 173–184.
Yuliatun S, Santoso EM. 2022. Pengaruh konsentrasi natrium hidroksida pada isolasi selulosa dari ampas tebu. Indonesian Sugar Research Journal 2(1): 12–21.
Dinev T, Tzanova M, Velichkova K, Dermendzhieva D, Beev G. 2021. Antifungal and antioxidant potential of methanolic extracts from Acorus calamus, Chlorella vulgaris Beijerinck, Lemna minuta Kunth and Scenedesmus dimorphus (Turpin) Kützing. Applied Sciences (Switzerland) 11(11): 1-13.
Febia A, Mukarlina, Rahmawati. 2020. Aktivitas antifungi ekstrak metanol daun bandotan (Ageratum conyzoides) terhadap Phytophthora sp. (Im5) secara in vitro. Jurnal Protobiont 9(2): 167–174.
Fitria F, Suhartini S, dan Prihandono DS. 2023. Gambaran kapang Aspergillus Sp. pada terasi dalam kemasan tanpa merek di pasar tradisional kota Samarinda. JI-KES (Jurnal Ilmu Kesehatan) 6(2): 147–154.
Hanif A, Zamriyetti Z. 2023. Karakterisasi morfologi cendawan penyebab penyakit busuk pangkal batang pada bawang merah (Allium cepa). AGRIUM: Jurnal Ilmu Pertanian 26(1): 76–82.
Julyeda NKD, Darmayasa, IBG, Hardini J. 2021. Potential of awar-awar leaf methanol extract (Ficus septica Burm. f.) against Aspergillus niger on Balinese lontar. Metamorfosa: Journal of Biological Sciences 8(2): 268-274.
Mohammed GJ, Hameed IH. 2018. Anti-fungal, antitumor and anti-inflammatory activity of Acorus calamus. Indian Journal of Public Health Research and Development 9(3): 254–258.
Nasardin NR, Zainon M, Zulkefle A, Hanafiah MA, Ibrahim M, Rahman AI. 2018. Comparative study on steam distillation and hydro-distillation methods for agarwood oil extraction. International Journal of Applied Engineering Research 13(8): 6253–6256.
Paranatha KAD, Bratayadnya PA, dan Octaviano AL. 2022. Konservasi Naskah lontar dalam fotografi story. Retina Jurnal Fotografi 2(1): 123–132.
Parwanayoni S, Sudirga SK. 2020. Isolasi dan identifikasi senyawa antijamur daun jeringau (Acorus calamus Linn.) sebagai pengendali jamur Athelia rolfsii Sacc. penyebab penyakit busuk batang pada tanaman kedelai. Metamorfosa: Journal of Biological Sciences 7(2):10-12.
Politeo O, Popovic M, Versic Bratincevic M, Koceic P, Nincevic T, Mekinic IG. 2023. Conventional vs. microwave-assisted hydrodistillation: influence on the chemistry of sea fennel essential oil and its by-products. Plants 12(7): 32-43.
Putri D, Nasir N, Agustien A. 2019. Kemampuan jamur akar putih (Rigidoporus microporus (Swartz: Fr.) Van Ov.) dalam mendegradasi limbah selulosa. Metamorfosa: Journal of Biological Sciences 6(1): 102-114.
Rampa E, Manurun S, Sinaga H. 2023. Isolasi fungi endofit dari buah dan daun belimbing wuluh. Jurnal Penelitian Kesehatan Suara Forikes 14(1): 92–96.
Rita WS, Kawuri R, Swantara IMD. 2017. The essential oil contents of jeringau (Acorus calamus L.) rhizomes and their antifungal activity against Candida albicans. Journal of Health Sciences and Medicine 1(1): 33-41.
Rohmah IN, Alif T. 2021. Uji pengembangan spora entomopatogen bunga entomopatogen Lecanicillium lecanii menggunakan haemocytometer. Jurnal Matematika Dan Sains 1(2): 143–150.
Sharma V, Sharma R, Gautam DNS, Kuca K, Nepovimova E, Martins N. 2020. Role of vacha (Acorus calamus Linn.) in neurological and metabolic disorders: Evidence from ethnopharmacology, phytochemistry, pharmacology and clinical study. Journal of Clinical Medicine 9(4): 67-74.
Simanjuntak HA, Butar M. 2019. Uji aktivittas antifungi ekstrak etanol umbi bawang merah (Allium Cepa L.) terhadap Candida albicans dan Pityrosporum Ovale. Eksakta: Jurnal Penelitian dan Pembelajaran MIPA 4(2): 91-98.
Sintawarak P, Uthairatsamee S, Keawgrajang T. 2021. In vitro and in vivo inhibition of Cylindrocladium reteaudii by essential oils of Acorus calamus rhizomes. Environment and Natural Resources Journal 19(1): 34–42.
Szczeblewski P, Wroblewski M, Borzyszkowska-Bukowska J, Bairamova T, Górska J, Laskowski T, Samulewicz, A, Kosno, M., Sobiech, Ł, Polit JT, Kukula-Koch W. 2022. The role of centrifugal partition chromatography in the removal of β-asarone from Acorus calamus essential oil. Scientific Reports 12(1): 1–14.
Tancinova D, Maskova Z, Foltinova D, Stefanikova J, Arvay J. 2018. Effect of essential oils of lamiaceae plants on the Rhizopus spp. Journal of Food Sciences 12(1): 491–498.
Venkatesan R, Karuppiah PS, Arumugam G, Balamuthu K. 2019. β-Asarone exhibits antifungal activity by inhibiting ergosterol biosynthesis in Aspergillus niger ATCC 16888. Proceedings of the National Academy of Sciences India Section B - Biological Sciences 89(1): 173–184.
Yuliatun S, Santoso EM. 2022. Pengaruh konsentrasi natrium hidroksida pada isolasi selulosa dari ampas tebu. Indonesian Sugar Research Journal 2(1): 12–21.
Diterbitkan
2024-06-29
##submission.howToCite##
INDI SWARI, Komang Kartika; DARMAYASA, Ida Bagus Gede; WIBAWA, I Putu Agus Hendra.
Penentuan Minimum Inhibitory Concentration (MIC) minyak atsiri Acorus calamus terhadap Aspergillus flavus.
Jurnal Biologi Udayana, [S.l.], v. 28, n. 1, p. 102-110, june 2024.
ISSN 2599-2856.
Tersedia pada: <http://103.29.196.112/index.php/bio/article/view/114545>. Tanggal Akses: 04 mar. 2026
doi: https://doi.org/10.24843/JBIOUNUD.2024.v28.i01.p09.
Bagian
Articles
