Eksplorasi dan uji aktivitas antimikroba jamur endofit dari daun mangga kasturi (Mangifera casturi Kosterm.) terhadap Escherichia coli ATCC 25922, Candida albicans ATCC 10231 dan Staphylococcus aureus ATCC 25923
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https://doi.org/10.24843/JBIOUNUD.2024.v28.i01.p07
Abstrak
The kasturi mango (Mangifera casturi Kosterm.) is categorized as an endangered plant in situ or Extinct in the Wild according to the IUCN Red List Categories, This also puts under threat the existence of endophytic fungi in these plants. This study aimed to isolate, characterize, and assay the antimicrobial activity of endophytic fungi from mango leaves toward Escherichia coli, Staphylococcus aureus, and Candida albicans. The fungi were screened for antimicrobial activity using the agar diffusion method. Antimicrobial activity was assessed by measuring the inhibition zone produced by endophytes against the pathogenic microbes. A total of 23 isolates belonging to 4 genera and 1 group of fungi were successfully isolated. Fungi belonging to the genus Aspergillus with a total of 13 isolates were dominant compared to Penicillium, Cladosporium, Syncephalastrum, and the Mycelia sterilia group. All isolates were able to inhibit the growth of S. aureus with the inhibition zone ranging from 13-23 mm, which is higher than Ampicillin 10 µg/ml. The endophytic fungi obtained were more effective at inhibiting the growth of S. aureus than E. coli, but lacked the ability to inhibit C. albicans.
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Referensi
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Nguyen VB, Nguyen AD, Kuo Y-H, Wang S-L. 2017. Biosynthesis of α-Glucosidase Inhibitors by a Newly Isolated Bacterium, Paenibacillus sp. TKU042 and Its Effect on Reducing Plasma Glucose in a Mouse Model. Int. J. Mol. Sci. 18(700): 1-12. DOI: 10.3390/ijms18040700
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Pasappa N, Pelealu JJ, Tangapo AM, Studi P, Fmipa B, Manado U. 2022. Isolation and antibacterial test of endophytic fungi from mangrove plant Soneratia alba on the coast of Manado city. Jurnal Pharmacon. 11: 1430–1437
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Santos D, Prazeres I, Silva D, Luís Cláudio Nascimento, da Silva MV, de Araújo JM, Cavalcanti M da S, Lima VL de M. 2015. Antibacterial activity of endophytic fungi from leaves of Indigofera suffruticosa Miller (Fabaceae). Frontiers in Microbiology 6(MAY): 1–7. DOI: 10.3389/fmicb.2015.00350.
Silva DPD, Cardoso MS, Macedo AJ. 2022. Endophytic Fungi as a Source of Antibacterial Compounds: A Focus on Gram-Negative Bacteria. Antibiotics 11(11): 1509-1564. DOI: 10.3390/antibiotics11111509.
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Strobel G, Daisy B. 2003. Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews 67(4): 491–502. DOI: 10.2307/1592233
Suhendar U, Mahyuni S, Sogandi S. 2021. Identification of molecular bacterial isolate endofit bacteria Kasturi mango (Mangifera casturi Kosterm) leaves and analysis of antibacterial activity. Jurnal Sains Natural 11(1): 24-29. DOI: 10.31938/jsn.v11i1.294
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Talukdar R, Padhi S, Rai AK, Masi M, Evidente A, Jha DK, Cimmino A, Tayung K. 2021. Isolation and characterization of an endophytic fungus colletotrichum coccodes producing tyrosol from Houttuynia cordata Thunb. using ITS2 RNA secondary structure and molecular docking study. Frontiers in Bioengineering and Biotechnology 9(June): 1–12. DOI: 10.3389/fbioe.2021.650247.
Tilwari A, Dixit P. 2018. Isolation and screening of endophytic fungus from medicinal plant Saraca asoca for antibacterial activity. Journal of Pharmacognosy and Phytochemistry (2004): 351–354.
Tiwari P, Bae H. 2022. Endophytic Fungi: Key Insights, Emerging Prospects, and Challenges in Natural Product Drug Discovery. Microorganisms 10(2): 360-403 DOI: 10.3390/microorganisms10020360
Vieira WAS, Michereff SJ. 2014. Endophytic species of Colletotrichum associated with mango in northeastern Brazil. Fungal Diversity. 67: 181–202. DOI: 10.1007/s13225-014-0293-6
Viogenta P, Nurjanah S, Mulyani YWT. 2020. Isolasi jamur endofitik rumput mutiara (Hedyotis corymbosa (L.) Lamk.) dan analisis potensi sebagai antimikroba. Jurnal Pharmascience 7(1): 72 – 83. DOI: 10.20527/jps.v7i1.8076
Watanabe T. 2002. Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species. Choice Reviews Online. CRC Press LLC: Boca Raton. DOI: 10.5860/choice.40-3398.
Yang EF, Karunarathna SC, Tibpromma S, Stephenson SL, Promputtha I, Elgorban AM, Al-Rejaie S, Chomnunti P. 2023. Endophytic fungi associated with mango show in vitro antagonism against bacterial and fungal pathogens. Agronomy 13(1): 72-83. DOI: 10.3390/agronomy13010169
Yu Z, Ding H, Shen K, Bu F, Newcombe G, Liu H. 2021. Foliar endophytes in trees varying greatly in age. Eur J Plant Pathol. 160: 375–384. DOI: 10.1007/s10658-021-02250-7.
Zaunit MM, Verawati, Fera O, Zalri DF. 2022. Isolasi dan identifikasi bakteri endofit dari daun pauh (Mangifera sumatrana Miq.) serta uji aktivitas antimikroba. Jurnal Katalisator 7(2): 178–191.
Alvin A, Miller KI, Neilan BA. 2014. Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds. 169: 483–495. DOI: 10.1016/j.micres.2013.12.009
Barnett H, Hunter BB. 1998. Illustrated Genera of Imperfect Fungi. Genera Fungi. APS Press: United States
Bhattacharya S, Sae-Tia S, Fries BC. 2020. Candidiasis and mechanisms of antifungal resistance. Antibiotics 9(6): 1–19. DOI: 10.3390/antibiotics9060312
Clinical and Laboratory Standards Institute (CLSI). 2021. Performance Standards for Antimicrobial Susceptibility Testing. 31st ed. CLSI supplement M100. Clinical and Laboratory Standards Institute, USA,
Dashyal MS, Sangeetha CG, Appanna V. 2019. Isolation and Morphological Characterization of Endophytic Fungi Isolated from Ten Different Varieties of Mango. Int. J. Curr. Microbiol. App. Sci. 8(03): 717–726
Dugan LM, Campbell PH, Wilcox MJ. 2006. Making Decisions About Assistive Technology With Infants and Toddlers. Topics in Early Childhood Special Education 26(1): 25–32. DOI: 10.1177/02711214060260010301
Elfina D, Martina A, Roza RM. 2014 Isolasi dan karakterisasi fungi endofit dari kulit buah manggis (Garcinia mangostana L) sebagai antimikroba terhadap Candida albicans, Staphylococcus aureus dan Escherichia coli. Jurnal Online Mahasiswa Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau 1.1: 1-10
Ellis MB. Ellis JP. 1988. Microfungi on Land Plants: an identification handbook. Cambridge University Press.
Gaganaa SL, Kumaraswamyb BE, Shivanna MB. 2020. Diversity, antibacterial and antioxidant activities of the fungal endophytes associated with Schleichera oleosa (Lour.) Merr. South African Journal of Botany 134: 369-381.
Gandjar I, Rifai MA. 1999. Pengenalan kapang tropik umum. Yayasan Obor Indonesia: Jakarta
Ichikawa T, Date M, Ishikura T, Ozaki A. 1971. Improvement of kasugamycin-producing strain by the agar piece method and the prototroph method. Folia microbiologica 16(3): 218–224. DOI: 10.1007/BF02884210.
Jubeh B, Breijyeh Z, Karaman R. 2020. Resistance of gram-positive bacteria to current antibacterial agents and overcoming approaches. Molecules 25(12). DOI: 10.3390/molecules25122888.
Katzung BG. 2012. Farmakologi dasar dan klinik edisi 10. Jakarta: EGC. EGC: Jakarta
Lestari Arum LD, Noverita, Permana A. 2020. Daya hambat propolis terhadap bakteri Staphylococcus aureus dan Escherichia coli. Jurnal Pro-life 7(3): 50–237
Manganyi MC, Ateba CN. 2020. Untapped Potentials of Endophytic Fungi: A Review of Novel Bioactive Compounds with Biological Applications. Microorganisms 8(12): 1-25. DOI: 10.3390/microorganisms8121934
Maurya VK, Kachhwaha D, Bora A, Khatri PK, Rathore L. 2019. Determination of antifungal minimum inhibitory concentration and its clinical correlation among treatment failure cases of dermatophytosis. J Family Med Prim Care 8(8): 2577-81.
Nguyen VB, Nguyen AD, Kuo Y-H, Wang S-L. 2017. Biosynthesis of α-Glucosidase Inhibitors by a Newly Isolated Bacterium, Paenibacillus sp. TKU042 and Its Effect on Reducing Plasma Glucose in a Mouse Model. Int. J. Mol. Sci. 18(700): 1-12. DOI: 10.3390/ijms18040700
Nia R, Mia M, Oktapiana K. 2017. Antibacterial activity test of endophytic fungus from mangrove plant (Rhizophora apiculata L.) and (Bruguiera gymnorrizha (L.) Lamk.) against Klebsiella pneumoniae ATCC 700603. KnE Life Sciences 2(6): 146. DOI: 10.18502/kls.v2i6.1031
Nuraini FR, Setyaningsih R, Susilowati A. 2019. Antioxidant activity of bioactive compound produced by endophytic fungi isolated from endemic plant of South Kalimantan Mangifera casturi Kosterm. AIP Conference Proceedings 2120(July). DOI: 10.1063/1.5115751
Nuraini FR, Setyaningsih R, Susilowati A. 2023. Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, Indonesia. Indonesian Journal of Biotechnology 28(2): 77‐85.
Nwakanma C, EN N, T P. 2016. Antimicrobial Activity of Secondary Metabolites of Fungi Isolated from Leaves of Bush Mango. Journal of Next Generation Sequencing & Applications 3(2): 1-6. DOI: 10.4172/2469-9853.1000135.
Pasappa N, Pelealu JJ, Tangapo AM, Studi P, Fmipa B, Manado U. 2022. Isolation and antibacterial test of endophytic fungi from mangrove plant Soneratia alba on the coast of Manado city. Jurnal Pharmacon. 11: 1430–1437
Ravimannan N, Arulanantham R, Pathmanathan S, Niranjan K. 2014. Alternative culture media for fungal growth using different formulation of protein sources. Scholars Research Library 5(1): 36–39. DOI: 0976-1233
Rhodes L, Maxted N. 2016. Mangifera, C. The IUCN red list of threatened species. https//www.iucnredlist.org
Santos D, Prazeres I, Silva D, Luís Cláudio Nascimento, da Silva MV, de Araújo JM, Cavalcanti M da S, Lima VL de M. 2015. Antibacterial activity of endophytic fungi from leaves of Indigofera suffruticosa Miller (Fabaceae). Frontiers in Microbiology 6(MAY): 1–7. DOI: 10.3389/fmicb.2015.00350.
Silva DPD, Cardoso MS, Macedo AJ. 2022. Endophytic Fungi as a Source of Antibacterial Compounds: A Focus on Gram-Negative Bacteria. Antibiotics 11(11): 1509-1564. DOI: 10.3390/antibiotics11111509.
Singh A, Singh D, Kharwar R, Gond S, White J. 2021. Fungal Endophytes as Efficient Sources of Plant-Derived Bioactive Compounds and Their Prospective Applications in Natural Product Drug Discovery: Insights, Avenues, and Challenges. Microorganisms 9(1): 197-244. DOI: 10.3390/microorganisms9010197.
Strobel G, Daisy B. 2003. Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews 67(4): 491–502. DOI: 10.2307/1592233
Suhendar U, Mahyuni S, Sogandi S. 2021. Identification of molecular bacterial isolate endofit bacteria Kasturi mango (Mangifera casturi Kosterm) leaves and analysis of antibacterial activity. Jurnal Sains Natural 11(1): 24-29. DOI: 10.31938/jsn.v11i1.294
Sukertiasih NK, Megawati F, Meriyani H, Sanjaya DA. 2021. Studi Retrospektif Gambaran Resistensi Bakteri terhadap Antibiotik. Jurnal Ilmiah Medicamento 7(2): 108–111. DOI: 10.36733/medicamento.v7i2.2177
Sukmana B, Edyson, Thahir H, Achmad H, Huldani, Bokov DO. 2020. Research review on secondary metabolite compounds of Mangifera casturi bark and their functions. International Journal of Pharmaceutical Research 12: 2155–2161. DOI: 10.31838/ijpr/2020.12.03.309.
Talukdar R, Padhi S, Rai AK, Masi M, Evidente A, Jha DK, Cimmino A, Tayung K. 2021. Isolation and characterization of an endophytic fungus colletotrichum coccodes producing tyrosol from Houttuynia cordata Thunb. using ITS2 RNA secondary structure and molecular docking study. Frontiers in Bioengineering and Biotechnology 9(June): 1–12. DOI: 10.3389/fbioe.2021.650247.
Tilwari A, Dixit P. 2018. Isolation and screening of endophytic fungus from medicinal plant Saraca asoca for antibacterial activity. Journal of Pharmacognosy and Phytochemistry (2004): 351–354.
Tiwari P, Bae H. 2022. Endophytic Fungi: Key Insights, Emerging Prospects, and Challenges in Natural Product Drug Discovery. Microorganisms 10(2): 360-403 DOI: 10.3390/microorganisms10020360
Vieira WAS, Michereff SJ. 2014. Endophytic species of Colletotrichum associated with mango in northeastern Brazil. Fungal Diversity. 67: 181–202. DOI: 10.1007/s13225-014-0293-6
Viogenta P, Nurjanah S, Mulyani YWT. 2020. Isolasi jamur endofitik rumput mutiara (Hedyotis corymbosa (L.) Lamk.) dan analisis potensi sebagai antimikroba. Jurnal Pharmascience 7(1): 72 – 83. DOI: 10.20527/jps.v7i1.8076
Watanabe T. 2002. Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species. Choice Reviews Online. CRC Press LLC: Boca Raton. DOI: 10.5860/choice.40-3398.
Yang EF, Karunarathna SC, Tibpromma S, Stephenson SL, Promputtha I, Elgorban AM, Al-Rejaie S, Chomnunti P. 2023. Endophytic fungi associated with mango show in vitro antagonism against bacterial and fungal pathogens. Agronomy 13(1): 72-83. DOI: 10.3390/agronomy13010169
Yu Z, Ding H, Shen K, Bu F, Newcombe G, Liu H. 2021. Foliar endophytes in trees varying greatly in age. Eur J Plant Pathol. 160: 375–384. DOI: 10.1007/s10658-021-02250-7.
Zaunit MM, Verawati, Fera O, Zalri DF. 2022. Isolasi dan identifikasi bakteri endofit dari daun pauh (Mangifera sumatrana Miq.) serta uji aktivitas antimikroba. Jurnal Katalisator 7(2): 178–191.
Diterbitkan
2024-06-29
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RAZAK, Indira Rahmana; MARTINA, Atria.
Eksplorasi dan uji aktivitas antimikroba jamur endofit dari daun mangga kasturi (Mangifera casturi Kosterm.) terhadap Escherichia coli ATCC 25922, Candida albicans ATCC 10231 dan Staphylococcus aureus ATCC 25923.
Jurnal Biologi Udayana, [S.l.], v. 28, n. 1, p. 76-89, june 2024.
ISSN 2599-2856.
Tersedia pada: <http://103.29.196.112/index.php/bio/article/view/101686>. Tanggal Akses: 04 mar. 2026
doi: https://doi.org/10.24843/JBIOUNUD.2024.v28.i01.p07.
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