Penyembuhan Luka Sayat Pada Kulit Tikus Putih (Rattus norvegicus) yang Diberi Ekstrak Daun Kirinyuh (Chromolaena odorata)

Abstrak

       Kirinyuh leaves (Chromolaena odorata)  are traditionally used for wound curing by the community. Kirinyuh leaves contains alkaloids, flavonoids, phenol, saponin, triterpenoid and tannin. The purpose of this study was to determine  the effect of kirinyuh leaves  extract on wound healing process in rat (Rattus norvegicus). This study used the completely randomized design, consisting of  5 treatments with 6 replicates. Thirty  white  rats with weight ranged of 200-250 g were used.  The mice were divided into five  treatments, namely  K- (aquades), K+ (povidione iodine 10 %), treatments (P1, P2, P3) is given extract of leaf kirinyuh 10%, 20%, 30%. Macroscopic observations were made on days 3, 6 and 9 for hyperemia, wound contraction, granulation, crusting,pus production. Preparation for microscopic observation (epidermal thickness, number of fibroblasts and amount of collagen) was made on day 10. Macroscopic data other than wound contraction were analyzed descriptively. Data on wound contraction, epidermal thickness, number of fibroblasts and the number of collagen were analysed using ANOVA (p<0.05) and if there is a significant difference, then proceed with Duncan test. The observation on macroscopic wound healing showed that the optimal 20% concentration in reducing wound hyperemia and contractions.  While the 10% concentration is optimal in the formation of granulation, preventing crusting and pus production.  Observations on microscopic wound healing showed that the optimal concentration of 20% (P2) was the most optimal in increasing epidermal thickness, increasing the number of fibroblasts and the amount of collagen.  It can be concluded that kirinyuh leaf extract can heal wounds optimally with different concentrations at each stage of healing.

Keywords: Chromoelana odorata, secondary metabolites, wound healing, Rattus norvegicus